Fig 1: Analysis of Hspb1 expression in TSC complex subunit 2-deficient kidney tumor cells (MKOC). (A) MKOC cells were treated with 20 nM Rapa or vehicle only (DMSO) for 48 h. Cell proliferation was then analyzed by XTT assays. Data are representative of three independent experiments. Each experiment was performed with three independent culture wells. The data are presented as the mean + SD. ***P<0.001 compared with Rapa. (B) MKOC cells were treated with 20 nM Rapa or vehicle only (DMSO) for 24 h. Total cell lysates were analyzed by western blotting with the indicated antibodies (Hspb1, pHspb1, pS6, S6 and ßactin). (C) MKOC cells were treated with 20 nM Rapa or vehicle only (DMSO) for 24 h. Total cell lysates were analyzed by western blotting with the indicated antibodies (Hspb1, Hsp90, Hsp70 and a-tubulin). Hsp90, heat shock protein 90; Hsp70, heat shock protein 70; Hspb1, heat shock protein family B (small) member 1; MKOC, MKOC1-277; p, phosphorylated; Rapa, rapamycin.